We have written code for Biomek NXp liquid handlers to make batches of 96 3’ RNAseq libraries using Lexogen's QuantSeq kit.
This code performs all pre-PCR steps in the protocol and requires manual intervention for incubation steps at or above 30 C. Optimization will be required for your local instrument.
This code is released under the terms of the following License.
The code is available here
A publication on the results produced from this method is now available at https://www.nature.com/articles/nature25966.